#FLOWJO COMPENSATION MATRIX IMPORT ZIP FILE#
#FLOWJO COMPENSATION MATRIX IMPORT ARCHIVE#

This difference is not expected to vary outside biological significance.

Statistics calculated on populations in FlowJo will differ from those calculated in FACSDiva™ for the same populations.

In other words, the experiment’s FCS files need to match.

Parameter naming is important for the conversion of FlowJo’s gates into FACSDiva™ (and vice versa).

We added a checkbox to out Diva importer to swap these back if you need that to be performed. This issue only occurs when data is exported from Diva “As Experiment.” If you export the data “As FCS” files, your data will export correctly.

When the data is exported from FACSDiva™ v8, the FSC-H and FSC-W values are swapped, as well as the SSC-H and SSC-W parameters.

Only the following gating types are supported for the conversion: Range, Bi-sector, Quad, Polygon, Rectangle, and Ellipses. Note: Ellipses converted into FACSDiva™ XML will turn into polygons to approximate the ellipsoid shape.

Make sure you’ve properly scaled parameters prior to gating in FlowJo, by accessing the Customize Axis dialog in the “T” button next to either axis in Graph Windows before gating. Only the following transformations are supported: Lin, Log, and Biex.Once you have made your selections, press the “Export to FACSDiva™” button to create the Folder containing the FACSDiva™ experiment XML.

Apply compensation and scaling to all files in experiment.

Choose the plot style foe the new plots.

Select the output directory for the new experiment.

Select the FACSDiva™ experiment to modify.

Create a new experiment or modify an existing experiment.

Simply right-click on a sample that contains the desired population hierarchy and gating, and from that dialogue, click on “Export to FACSDiva™.” A new window will open with the following options: When you have created the gating structure in FlowJo v10.6+ needed for repeating your experiment, you can modify an existing Diva experiment (.XML file) that and import that file into FACSDiva™.

Whether you would like to import compensation and scaling onlyĪfter making these decisions, you will then need to click the “Import from FACSDiva™” button to bring the information into FlowJo.

An FCS file with the scaling and compensation that you would like applied.

The Global Worksheet with the gates that you are importing.

The experiment using the file navigator.

Once the Import from FACSDiva™ dialogue has been opened, you will be asked to choose: Next, right-click on any of loaded samples and select “Import from FACSDiva™” to open the Import from FACSDiva™ dialogue. With FlowJo v10.6 open, load your FCS files from the experiment by dropping the files into the FlowJo Workspace or from the Add Samples dialogue. Importing FACSDiva™ Experiments into FlowJo

#FLOWJO COMPENSATION MATRIX IMPORT ARCHIVE#

Choosing Directory Export will allow for the direct import into FlowJo, as the Zip archive will need to be unzipped first.

#FLOWJO COMPENSATION MATRIX IMPORT ZIP FILE#

XML Choosing Zip File Export will export a Zip archive file that contains your experiment. You will be given the option to export as a Directory or a Zip File.Ĭhoosing Directory Export will export a folder with your experiment.

XML) by either right-clicking on the experiment and selecting Export –> Experiments from the dialogue or by choosing File –> Export –> Experiment on the FACSDiva™ ribbon. Once you have finished collecting your flow cytometry data on a BD cytometer running FACSDiva™, you will need to export your experiment file (FACSDiva™ experiments have a file extension of. Additionally, you can now create FACSDiva™ experiments in FlowJo for experiment reiterations and precise cell sorting gates. This means that gating information and population hierarchies don’t need to be lost in the handoff. Now, with FlowJo v10.6 and newer, FACSDiva™ experiments can be imported into FlowJo and FlowJo can export FACSDiva™ templates. Until now, FCS files were the only data from FACSDiva™ that could be imported into FlowJo. Since 1994, Flow Cytometrists have been using FlowJo to analyze data collected on BD cytometers to create statistics and figures pivotal to publications on immune subsets.

Flowjo compensation matrix import